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Figure 5
The effect of regulatory subunits (MATβ) and SCR0911 on the activity of MATα1 R264H. (a) Gel-filtration profiles of MATα1 R264H–MATβV1 with or without SCR0911 pre-incubation are shown by the absorbance at A280. (b) The effects of the regulatory subunits (MATβV1/V2) and SCR0911 on the enzymatic activity of MATα1 R264H are shown by measuring SAMe formation. `aaa', `bbb' and `ccc' denote statistical significance at p < 0.001 compared with tetrametric R264H, dimeric R264H (dimer) and both oligomeric forms of R264H, respectively. Data are the mean ± SEM (n = 3). (c) The SCR0911 binding site of the R264H mutant (grey) obtained by molecular docking is aligned with the structure of the complex of MATα2 (yellow) and MATβ (pink). SCR0911 is shown as blue sticks. (d) The SCR0911 and R264H interaction profiles obtained from SwissDock are illustrated. Protein–ligand interactions are shown in black for hydrogen bonds, blue for halogen bonds, pink for ππ stacking and orange for electrostatic interactions.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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