Figure 4
Soaking of apo crystals of Xcc4156. Soaks were performed by the addition of 0.5 µl 150 mM NaBr (`NaBr'), 15 mM FAD ('FAD'), 15 mM FAD and 150 mM NaBr (`FAD–NaBr') or 200 mM NaBr (`NaBr200') in reservoir solution to a drop consisting of 0.5 µl reservoir solution and 1 µl protein solution. Photographs were taken directly after the addition of the ligand solution to the drop (`0 h') and after 24 h. The pink bar indicates 100 µm. |