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![[Figure 1]](ih5002fig1mag.jpg)
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Figure 1
Fringe-free illumination. (a, b) Image of the beam without (a) and with (b) fringe-free illumination. The beam diameter is 460 nm and the images were recorded at spot 9 over a 20 s exposure with a pixel size of 1.34 Å. (c, d) Acquisition scheme without (c) and with (d) fringe-free illumination. The sample is embedded in a thin layer of ice over a holey carbon film with 2 µm diameter holes. The blue squares represent the imaged area in the context of a 1.04 Å pixel. Without fringe-free illumination (c) the beam size, depicted by an orange circle, needs to be ∼1 µm in diameter in order to avoid seeing fringes within the imaged area, limiting the number of acquisitions to five within the hole. In the case of fringe-free illumination (d), a beam size of 600 nm is enough to cover the camera, allowing up to ten acquisitions. |
![[Figure 1]](ih5002fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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