Figure 5
Bromide anomalous signal in lysozyme KBr soaks. Anomalous maps were calculated using ANODE (Thorn & Sheldrick, 2011). The anomalous signal of bromide is displayed at 10σ for diffraction data collected at 100 K (blue) and at 3.5σ for RT data (magenta). The lysozyme backbone is represented as a green cartoon. Black arrows represent anomalous peaks common to the RT and 100 K data sets. The sigma levels were chosen to have strong visible peaks above the noise level. This figure was created using PyMOL (DeLano, 2008). |