Figure 6
Predicted association of Rac1 with CYRI-B and CYFIP1. (a) Site-directed mutagenesis data mapped onto the R. typus CYRI-B crystal structure. Mutations in CYRI-B (black labels) that impair (red spheres) or do not affect (blue spheres) its function in vivo (Shang et al., 2018 ) are shown. Green labels indicate mutations in the CYFIP1 DUF1394 domain that perturb the association of the WRC complex with Rac1 (Chen et al., 2010 ). (b) Close-up views of the predicted Rac1-interacting regions of CYRI-B (left) and CYFIP1 (right), coloured as in Fig. 5 . Residues leading to effective mutations in (a) are represented as cyan sticks with the corresponding mutated residue indicated. Electron densities of CYRI-B side chains are shown as a blue mesh contoured at a σ level of 1. |