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![[Figure 5]](nj5292fig5mag.jpg)
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Figure 5
Comparisons between the Met and Δ1L2M CXCL13 crystal structures. Structural features are labeled. Missing residues are shown as dashed lines. (a) The seven core domains from the Δ1L2M CXCL13 structure (shades of gray) as well as that from the Met CXCL13 structure (blue) were overlaid to demonstrate their rigidity (see Supplementary Fig. S3 for calculated r.m.s.d. values). Note that the β0 strands localized in the N-loop only occur in the Δ1L2M CXCL13 monomers. (b) The Met CXCL13 monomer was overlaid with a single Δ1L2M CXCL13 monomer (chain E) to demonstrate the differences in the positions of their N-termini. N1 and β0 #1 in the figure refer to structural features in Met CXCL13, while N2, β0 #2 and β−1 in the figure refer to structural features in Δ1L2M CXCL13. The red circle indicates the branch point of the N-termini, which occurs close to the C-X-C motif. (c) The same monomers as shown in (b) are used to demonstrate that the angle between the main-chain atoms upstream of the C-X-C motif in the two structures was approximately 124.1°. Note that the β1–β2 loop is hidden for clarity. (d) Alignment of all seven Δ1L2M monomers and the Met CXCL13 monomer (minus their N-termini, which are hidden) emphasizes that the C-terminal extension of CXCL13 is incredibly flexible (see also Fig. 4 ![[link]](../../../../../../logos/arrows/d_arr.gif) c). |
![[Figure 5]](nj5292fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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