Figure 10
Phase diagram of lysozyme crystallization in 15% PEG 4000 with various concentrations of lysozyme and sodium chloride. The crystallization condition without lysozyme and sodium chloride was 15% polyethylene glycol, 0.04% sodium azide in 50 mM sodium acetate buffer pH 4.5 using the batch method. The darkest blue circles indicate crystals that appeared within 3 h of crystallization setup. Darker blue circles indicate those that appeared within three days. Light blue circles indicate those that appeared within seven days. Lighter blue circles indicate those that appeared after seven days. White circles indicate no crystal growth. The line indicates the solubility of lysozyme. |