Figure 7
Time course of crystal growth. The batch solution volume was 2 µl 12 mg ml−1 lysozyme, 0.4 M sodium chloride, 15% PEG 4000, 0.04% sodium azide, 50 mM sodium acetate buffer pH 4.5. The initial crystal growth rate dL(t)/dt was extrapolated as 0.65 ± 0.06 µm h−1 based on the slope of the approximated straight line. The estimated nucleation start time was 126 ± 21 h from the horizontal intercept of this line. The photos are of the lysozyme crystal 241 h (left) and 855 h (right) after crystallization setup. |