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![[Figure 1]](nj5295fig1mag.jpg)
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Figure 1
(a) Domain schematic for the production of GHK-tagged protein. (b) Representation of Cu-GHK coordination adapted from the coordinates of the Cu-GHK crystal structure CCDC-809108 {https://dx.doi.org/10.5517/ccw4y86; [copper(II)(GHK)]}. (c) The copper coordination in GHK-GFP crystal form I; the 2Fo − Fc map is contoured at 1σ and the REFMAC5 anomalous difference map is contoured at 10σ. (d) MALS analysis of GHK-GFP performed in the presence of 1 mM CuSO4, showing a molecular weight of 2.700 × 104 kDa (±0.428%); the theoretical monomer molecular weight is 2.631 × 104 kDa. |
![[Figure 1]](nj5295fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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