Figure 2
(a) A comparison of the improvements in phase error as calculated with SFTOOLS before and after the inclusion of LLG map completion with residual sulfur sites and with density modification using Parrot. (b) The structure of GHK-GFP crystal form I is shown as a cartoon in grey. The GHK motif is highlighted in cyan. Native sulfur-containing residues are shown as sticks, with Phaser-refined S atoms from LLG maps shown as blue spheres. The REFMAC5 anomalous difference map is contoured at 4σ. For clarity only one chain is shown. |