Figure 9
The Shifter enables more crystals (NUDT7A) to be mounted from every drop. (a, b, c) Well drops containing multiple crystals were identified from images based on the number of easily accessible 35–75 µm crystals (green squares). 10–35 µm or inaccessible crystals were counted as second-choice targets, which might be of interest under real conditions (green diamonds). Microcrystals were not counted. The images shown in (a), (b) and (c) relate to experiment 1 in the `Shifter Stationary' series (Fig. 10, middle panel). (d) The X-ray diffraction limits were similarly distributed for crystals mounted from Manual and Shifter experiments. (e) Practical considerations limited us to one UniPuck per well (16 crystals). For Manual wells, the drops dried out before reaching this limit (red hatching). For the Shifter wells this quota was met, necessitating that the remaining viable crystals be abandoned (orange hatching). |