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![[Figure 1]](nj5293fig1mag.jpg)
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Figure 1
Schematic overlay of the crystallization phase diagrams for the three used model enzymes. The proposed boundaries between the metastable zone and the nucleation zone are drawn differently for ADC/AcNiR (dashed blue line a) and ECAO (dotted blue line b). The same generic boundary between the nucleation and the precipitation zone is drawn for ADC and ECAO (line c). AcNiR crystallizes from an initial precipitation phase; thus, there is no sharp boundary between the nucleation and precipitation zones, as indicated by the striped area in blue (labelled d). The grey arrows show the different ways of moving through the phase diagram. For ADC and ECAO the ammonium sulfate concentration was adjusted directly, resulting in a horizontal move along the phase diagram (*). For AcNiR this was performed by changing the protein:precipitant ratio, thereby moving on the diagonal (**). The vertical red/black arrows represent time spent in the metastable zone for batch crystallization at (1) a lower ammonium sulfate concentration for ADC (i), ECAO (ii) and AcNiR (iii) and (2) a higher concentration. The red part of the arrow illustrates the difference in time spent in the nucleation zone, depending on the starting point and the borderline between the metastable zone and the nucleation zone. For AcNiR no effect on crystal size could be observed, but effects on crystallization speed and the amount of accompanying amorphous precipitation were observed upon increasing the ammonium sulfate concentration. |
![[Figure 1]](nj5293fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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