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![[Figure 1]](nj5298fig1mag.jpg)
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Figure 1
Characterization of C-PC sample solutions prior to crystallization. (a) SDS–PAGE (12% acrylamide) analysis of the chromatographic fractions containing pure C-PC used in crystallization trials. (b) Averaged spectra of C-PC obtained by CD spectroscopy buffered at pH 8.0 (20 mM Tris; blue), pH 6.5 (20 mM MES; purple) and pH 4.0 (20 mM acetate; red), indicating a nearly identical secondary structure in all three buffers. (c) Autocorrelation functions of C-PC depending on the delay time as obtained by IR-DLS indicate an increased degree of C-PC solution polydispersity and additional larger particles at pH 4.0 compared with pH 8.0 and pH 6.5. |
![[Figure 1]](nj5298fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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