Figure 5
(a) Analysis of retinoid fluorescence of DC271 and DC645 in the presence and absence of CRABPII (n = 3). It can be seen that DC271 presents a stark solvatochromic effect when in the presence of CRABPII owing to inclusion into the binding site, as discussed previously (Chisholm et al., 2019). DC645 conversely displays some intrinsic fluorescence but no solvatochromic effect. (a) Binding analysis of DC645 to CRABPII by the displacement of DC271; curve fitting was performed using DynaFit (Kuzmič, 1996; Tomlinson et al., 2018). |