Crystal structures of the selenoprotein glutathione peroxidase 4 in its apo form and in complex with the covalently bound inhibitor ML162

Moosmayer, D.; Hilpmann, A.; Hoffmann, J.; Schnirch, L.; Zimmermann, K.; Badock, V.; Furst, L.; Eaton, J.K.; Viswanathan, V.S.; Schreiber, S.L.; Gradl, S.; Hillig, R.C.

doi:10.1107/S2059798320016125

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 2
(a) Comparison of the effect of different SECIS elements on GPX4 expression when co-expressed with rat SBP2 using transient transfection in HEK293-6E cells. Transfected cells were lysed and GPX4 was partially purified by IMAC, and the eluates were analyzed by SDS–PAGE. Lanes 1–4 show expression of GPX4 with the chimeric SECIS (lane 1), with SelN SECIS (lane 2), with GPX4 SECIS (lane 3) and with T. gondii SECIS (lane 4). Lane 5 shows Mock-transfected HEK293-6E cells. The arrow indicates the band representing GPX4^WT (molecular weight 21 155 Da). (b) Purification steps for GPX4^WT analyzed by SDS–PAGE. Lane 1 shows the pooled fractions after IMAC as subjected to gel filtration; lane 2 shows the pooled fractions of the final gel-filtration step. (c) Mass-spectrometric analysis of purified GPX4^WT is consistent with 100% incorporation of one Se atom. The deconvoluted spectrum is shown.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 77| Part 2| February 2021| Pages 237-248

https://doi.org/10.1107/S2059798320016125

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