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![[Figure 1]](ic5114fig1mag.jpg)
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Figure 1
Schematic overview of a typical workflow involved in MicroED. (a) Microcrystals are grown using sitting-drop vapour diffusion. When a crystallization drop is identified containing potential microcrystals suitable for MicroED, the drop is mixed with buffer solution and (b) deposited on a standard EM grid. Excess liquid is blotted away using filter paper, either from the back-side or both sides of the grid, and the grid is vitrified and cryo-transferred to the TEM. (c) In imaging mode, the grid can be screened for thin hydrated protein microcrystals that are suitable for data collection. (d) Switching to diffraction mode, MicroED data can be collected by continuously rotating the microcrystal about the rotation axis, effectively rotating the crystal lattice in reciprocal space, analogous to the rotation method in macromolecular X-ray crystallography. The diffraction patterns can then be indexed, the intensities are extracted and the structure can be determined by molecular replacement (e) followed by model building and structure refinement. |
![[Figure 1]](ic5114fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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