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![[Figure 3]](ic5114fig3mag.jpg)
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Figure 3
Typical crystal morphologies suitable for MicroED as seen under an optical microscope (top) and on the grid using TEM (bottom). (a) Needle-shaped nanocrystals of orthorhombic hen egg-white lysozyme; the crystals are 100–200 nm in thickness and several micrometres in length (Clabbers et al., 2017  ; Xu et al., 2018). (b) Needle-shaped dynamin GTPase microcrystals of 0.5–1.5 µm in diameter and several micrometres in length. (c) Triangular-shaped plate-like R2lox crystals; the crystals are less than 500 nm in thickness and a few micrometres in size (Xu et al., 2019). (d) Fragments of large HCA II crystals; the fragments are 1–2 µm in size with a thickness of less than 500 nm (Fisher et al., 2012; Clabbers et al., 2020). (e) Fragmented crystals of tetragonal lysozyme; individual fragments are around 1 µm in size (Barends et al., 2014). (f) Diamond-shaped R2c crystals of 2–15 µm in size. As the thinner edges of the crystals did not diffract well, MicroED data had to be collected from smaller crystals (Andersson & Högbom, 2009). |
![[Figure 3]](ic5114fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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