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![[Figure 2]](id5010fig2mag.jpg)
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Figure 2
Adherence of KBP to a GO substrate prevents partial denaturation. (a) Representative micrograph of KBP prepared on holey carbon grids coated with GO. GO, graphene oxide layered over holes in the carbon. Cb, carbon support. Scale bar = 40 nm. (b) Exemplar well populated 2D classes of KBP prepared on holey carbon grids coated with GO. (c) Comparison of well populated 2D classes of KBP prepared on holey carbon grids with or without a GO coating. (d) Selected 2D classes from data on holey carbon or GO on holey carbon (left top and bottom panels, respectively). These 2D classes appeared to be similar views of KBP, except with extra density in the 2D class on GO, as indicated with a dashed magenta line in the right-hand panel. (e) 3D reconstruction calculated from well populated KBP 2D classes from holey carbon grids (solid light blue density) superimposed onto a 3D reconstruction of well populated KBP 2D classes from GO-coated holey carbon grids (mesh). The magenta-colored mesh indicates extra density in the reconstruction from GO-coated holey carbon grids. (f) 3D reconstruction of well populated KBP 2D classes from GO-coated holey carbon grids. Semi-transparent magenta density indicates extra density as in (e). Modeled predicted helices are fitted (tubes), with all 19 now accounted for, including six helices that were only apparent in the reconstruction from well populated KBP 2D classes from GO-coated holey carbon grids (magenta tubes; compare with Fig. 1 ![[link]](../../../../../../logos/arrows/d_arr.gif) d). |
![[Figure 2]](id5010fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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