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![[Figure 3]](id5010fig3mag.jpg)
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Figure 3
KBP adopts preferred orientations on a GO substrate. (a) 90° rotated views of the 3D reconstruction of well populated KBP 2D classes from GO-coated holey carbon grids, illustrating anisotropic smearing. (b) 3D angular distribution (top, RELION output plot), Fourier space point-spread function (PSF) and efficiency Eod measure corresponding to the reconstruction and orientation shown in the bottom image in (a). The more circular the PSF is, the more isotropic the data are. Eod is a statistical measure characterizing the angular distribution, where a value of 1 is a perfectly isotropic angular distribution and a value of ∼0.6 is suboptimal (Naydenova & Russo, 2017  ). (c) Representative micrograph of KBP prepared on holey carbon grids coated with GO with 40° stage tilt applied. GO, graphene oxide layered over holes in the carbon. Cb, carbon support. Scale bar = 40 nm. |
![[Figure 3]](id5010fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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