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![[Figure 5]](id5010fig5mag.jpg)
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Figure 5
Combining KBP particles from GO-coated and GO-free grids allows the calculation of an isotropic 4.6 Å resolution reconstruction. (a) 90° rotated views of the 3D reconstruction of combined KBP-full 2D classes derived from both GO-coated and noncoated holey EM grids, exhibiting isotropic density. (b) 3D angular distribution (top, RELION output plot) and Fourier PSFs and Eod (below) for combined KBP-full particles derived from both GO-coated and noncoated (No GO) holey EM grids. The orientation of the 3D angular distribution and Fourier PSF correspond to the KBP reconstruction orientation shown at the bottom of (a) (and at the bottom of Fig. 3 ![[link]](../../../../../../logos/arrows/d_arr.gif) a). An Eod of >0.8 indicates a very good angular distribution (Naydenova & Russo, 2017). (c) Pie chart indicating the proportion of KBP-full particles contributing to the reconstruction in (a) from holey carbon, holey gold or holey carbon plus GO grids. (d) Gold-standard Fourier shell correlation (FSC) curves between independent masked, unmasked, phase-randomized and corrected half-maps (Chen et al., 2013) for the combined KBP-full data-set reconstruction shown in (a) calculated by RELION version 3.1 (Zivanov et al., 2018; 4.6 Å resolution at the `gold-standard' 0.143 FSC cutoff). (e) 90° rotated views of the KBP-full 3D reconstruction shown in (a) but with semi-transparent density and helices (as tubes) and loops of the fitted KBP model shown. Tube helices and their respective connecting loops are colored as in Figs. 1(d) and 2(f). Density for the magenta helices is absent in the KBP-partial species. |
![[Figure 5]](id5010fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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