journal menu
![[Figure 6]](id5010fig6mag.jpg)
|
|
Figure 6
KBP–KIF1A_MD sample preparation on GO reveals heterogeneity and flexibility. (a) SDS–PAGE gel of purified KBP–KIF1A_MD complexes (∼110 kDa in total). (b) Representative screening micrographs (at 120 kV without a VPP; see Section 2 ![[link]](../../../../../../logos/arrows/d_arr.gif) ) of KBP–KIF1A_MD complexes prepared on GO-coated holey carbon or gold grids, showing aggregation (white arrows) in the former case. GO, graphene oxide substrate. Scale bar = 40 nm. (c) Representative micrograph (data collection at 300 kV without a VPP) of KBP–KIF1A_MD complexes prepared on GO-coated holey gold grids. Scale bar = 40 nm. (d) Example 2D classes of dissociated KBP-full/partial/KIF1A_MD (left), KBP-partial–KIF1A_MD complexes (center) and KBP-full–KIF1A_MD complexes (right) derived from GO-coated holey gold grids. (e) Exemplar 2D classes of KBP-full–KIF1A_MD showing flexibility of the KIF1A_MD (blue false color) position and density blurring relative to KBP (pink false color), which has been roughly aligned between the different 2D classes. (e) Pie chart indicating the proportions of different species identified by 2D classification in the KBP–KIF1A_MD sample on holey gold plus GO grids. |
![[Figure 6]](id5010fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
access
