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![[Figure 1]](rr5201fig1mag.jpg)
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Figure 1
Hostal method. A guest protein (pink ovals) is introduced by diffusion (soaking) into a protein host crystal (dark blue hexagonal outlines), where it occupies the solvent channels (light blue filled hexagons). If guest-protein molecules populate the solvent channels to a sufficient extent and with high order, the guest-protein structure can in principle be solved using conventional structure-determination methods (upper path). If guest-protein molecules populate the solvent channels incompletely or with lower order (lower path), then recovering structural information for the guest protein is not trivial and requires future work, as indicated by the dotted arrows and text boxes. Non-ordered guest incorporation may be further explored for biotechnological applications (green). The present study investigates the incorporation of protein guests into a host crystal via soaking (yellow shading on the left). |
![[Figure 1]](rr5201fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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