Figure 3
Guest-protein uptake. The left panels show bright-field microscopy images of crystal droplets taken several days after the addition of solid lyophilized protein: (a) Texas Red-labeled CaM, (b) CytC. The upper right panels show a crystal ∼1 h after transfer to a fresh reservoir droplet lacking guest protein, with a larger nonsoaked crystal included for reference in (b). The lower right panels show soaked crystals (50–100 µm) mounted in LithoLoops and imaged at the goniometer using the microscope camera on beamline P13, DESY, Hamburg. |