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![[Figure 4]](rr5201fig4mag.jpg)
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Figure 4
Fluorescence emission spectra of crystals with UV excitation. (a) Emission spectra with excitation at 355 nm with the SP5-X microscope (top) or at 405 nm with the SP5 inverted microscope (bottom). Each spectrum is the result of averaging from four or five individual crystals of nonsoaked TrpR host (black solid lines) and crystals soaked for one day with CytC (red solid lines). Dotted lines show the emission from the surrounding solution in the microscope slide well (black, 4.5 mg ml−1 TrpR in reservoir buffer with 15.75% 2-propanol; red, 150 mg ml−1 CytC in reservoir buffer with 15.75% 2-propanol). (b) Normalized emission spectra of crystal samples (highest emission of the sample set to one and background emission set to zero). (c) Upper panels, a representative nonsoaked crystal; lower panels, a representative crystal soaked for one day with 150 mg ml−1 CytC. Images (left) were collected with the focal plane through the center of the crystal with 405 nm excitation (SP5 inverted microsope) and emission between 614 and 790 nm. Plots of fluorescence intensities along the indicated green dotted lines are shown to the right of each crystal image. |
![[Figure 4]](rr5201fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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