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![[Figure 5]](rr5201fig5mag.jpg)
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Figure 5
Host penetration. CLSM with excitation at 528 nm and emission at 550–598 nm was used to follow the progress of Alexa532-CaM penetration into host crystals. (a) Subsurface penetration. xyt scans taken with the focal plane set just below the surface of a single crystal, as shown in the left panel, at the indicated time points (right panel) after transfer of the crystal into reservoir solution containing 0.26 mg ml−1 (15 µM) Alexa532-CaM. (b) Depth of host penetration. z-stack scans for a representative single crystal as shown in the schematic in the left panel. Right panels, scans at successive z planes with 4 µm spacing of a microscope well containing many small crystals after one day of soaking. (c) Progression of host penetration. As in (b) after six days of soaking. (d) Soaking with high concentrations of CaM. CLSM images (left panels) and plots (right panels) show the fluorescence intensity from three representative crystals at the cross section indicated in the left panel at 5 min, 7 days, 15 days (from top to bottom) after soaking with ∼100 mg ml−1 CaM including 0.1% Alexa532-CaM. |
![[Figure 5]](rr5201fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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