Figure 5
Sequence conservation of the C-terminal loop and hook-loop. Sequence alignment of the C-terminal loop (a) and the hook-loop (b) of Prp2, Prp43, Prp16 and Prp22 in Chaetomium thermophilum, Saccharomyces cerevisiae, Homo sapiens, Xenopus laevis and Caenorhabditis elegans. (c) Helicase activities of various ctPrp43 constructs with a mutated C-terminal loop or/and hook-loop. An overview of the activities is shown as a bar plot and the kobs values are listed below. All experiments were performed in triplicate; the standard deviation is highlighted as error bars and indicated as ± in the table. |