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Figure 3
Size-exclusion chromatographic profile and calibration curve of VhGlcNAcase and standard proteins. (a) The HiPrep 26/60 Sephacryl S-300 prepacked column was calibrated with six well defined globular protein standards plus the small molecule Nɛ-DNP-L-lysine, ranging from 0.35 to 669 kDa. Nɛ-DNP-L-lysine (0.35 kDa) was used to estimate the internal volume of the column and blue dextran 2000 was used to determine the void fraction. (b) The estimated molecular mass of VhGlcNAcase (76 kDa, expected 74 kDa) was determined from the calibration plot of Kav versus log MW after the Kav value had been calculated from the measured elution volume.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 77| Part 5| May 2021| Pages 674-689
https://doi.org/10.1107/S2059798321002771
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