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Figure 1
Design of PA14-insertion sites in the PDZ tandem of AaRseP. (a) 3D structure of the PDZ tandem of AaRseP. The PDZ tandem structure (PDB entry 3wkm, chain A) is shown as a ribbon model with a transparent surface in two different views. Each PDZ domain is colored by its six β-strands. The α-­helices conserved in the PDZ fold are colored gray. The two PA14-insertion sites are highlighted with dotted circles. (b) Topology diagram. Both of the PDZ domains in AaRseP are circular permutants of the canonical PDZ fold. The first mutant was constructed by replacing residues 182–183 of the βF–βA loop of the PDZ-N domain with the PA14 tag. In the second mutant, PA14 was inserted between residues 235 and 236 of the βD–βE loop of the PDZ-C domain.

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ISSN: 2059-7983
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