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Figure 7
Antibody-assisted negative-staining EM analysis of PA14-inserted full-length AaRseP. (a) 3D reconstruction models of AaRseP (181-PA14-184) complexed with the NZ-1 Fab. Four different 3D reconstructions from the 2D class average images are shown in different colors. The numbers of particles used for the reconstruction are indicated below each model. (b) Superposition of the 3D reconstructions. The four models in (a) were aligned based on the putative AaRseP region. The NZ-­1 Fabs appear to contact AaRseP at almost the same point in each model. (c) 3D reconstruction model of AaRseP (235-PA14-236) complexed with the NZ-1 Fab. The most well averaged class is drawn as a representative.

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ISSN: 2059-7983
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