Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion

Tamura-Sakaguchi, R.; Aruga, R.; Hirose, M.; Ekimoto, T.; Miyake, T.; Hizukuri, Y.; Oi, R.; Kaneko, M.K.; Kato, Y.; Akiyama, Y.; Ikeguchi, M.; Iwasaki, K.; Nogi, T.

doi:10.1107/S2059798321002527

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 7
Antibody-assisted negative-staining EM analysis of PA14-inserted full-length AaRseP. (a) 3D reconstruction models of AaRseP (181-PA14-184) complexed with the NZ-1 Fab. Four different 3D reconstructions from the 2D class average images are shown in different colors. The numbers of particles used for the reconstruction are indicated below each model. (b) Superposition of the 3D reconstructions. The four models in (a) were aligned based on the putative AaRseP region. The NZ-1 Fabs appear to contact AaRseP at almost the same point in each model. (c) 3D reconstruction model of AaRseP (235-PA14-236) complexed with the NZ-1 Fab. The most well averaged class is drawn as a representative.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 77| Part 5| May 2021| Pages 645-662

https://doi.org/10.1107/S2059798321002527

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