Humidified glovebox to minimize dehydration risks during sample preparation and support loading and sealing. (a) Schematic illustration showing the glovebox components, with the stereo microscope and gas and vacuum connections removed. (b) Front view of the glovebox. A long working-distance binocular microscope rests on a heated window in a trough in the lid of the glovebox, allowing inspection of the sample-loading station and crystallization trays. (c) The glovebox holds the station (here shown with its enclosure removed), crystallization trays, buffer and cryoprotectant solutions, and other tools and supplies needed. Feedthroughs connect the station to vacuum and to a separate humidity-controlled gas stream. The fan-driven humidifier at the upper right raises the humidity within the glovebox from ambient humidity (typically <50% r.h.) to >95% r.h. in roughly 10 min.