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![[Figure 1]](cb5124fig1mag.jpg)
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Figure 1
Solution studies of the MtbLigA AdD domain (AdD) using small-angle X-ray scattering (SAXS). (a) Experimental scattering curve of the AdD–NMN complex (magenta). The DAMMIF fit (black line) and CRYSOL fit (green line) of the model generated based on SAXS density using PDB entry 1ta8 as a model are shown along with the χ2 value for fitting. (b) Experimental scattering curve of the AdD–NAD+ complex (blue). The DAMMIF fit (brown line) and CRYSOL fit of the model generated by elNémo based on SAXS density using PDB entries 1tae and 3sgi as models (green and pink lines for closed and partially closed, respectively). The χ2 values are also indicated on the graph. (c) Experimental scattering curve for the AdD–NMN+AMP complex (forest green). The DAMMIF (orange line) and CRYSOL fits of the model generated by elNémo based on the experimental density using PDB entry 6kjm as a model (black line) are shown along with the χ2 value for fitting. (d) Distance distribution P(r) curves for AdD–NMN (magenta), AdD–NAD+ (blue) and AdD–NMN+AMP (forest green). (e) Kratky plots for the AdD–NMN and AdD–NMN+AMP complexes show lower globularity than the AdD–NAD+ complex. (f) Guinier plot of all three complexes showing that the samples are devoid of any aggregation. (g–j) elNémo-generated models based on the SAXS density using PDB entries 1ta8, 1tae, 3sgi and 6kjm. |
![[Figure 1]](cb5124fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
