journal menu
![[Figure 3]](jc5035fig3mag.jpg)
|
|
Figure 3
CD and thermostability analysis of Sc-Rsm22. (a) CD spectra of the Sc-Rsm22 monomer and dimer. The CD spectra in the wavelength range 250–270 nm indicate that monomeric Sc-Rsm22 has more α-helical content than dimeric Sc-Rsm22 in 2.28 mM Tris pH 7.5, 28.5 mM NaCl, 0.28% glycerol. However, monomeric Sc-Rsm22 changes its structural fold in the direction of that of dimeric Sc-Rsm22 in the CD measurement solvent. The change in fold is rapid at the beginning of the solvent exchange from higher to lower salt concentration but stabilizes within 30 min. The figure shows the CD spectra of the Sc-Rsm22 monomer collected just after changing the solvent (first run, orange) and after 30 min (second run, blue) and of the Sc-Rsm22 dimer (gray). (b) The melting curve of dimeric Sc-Rsm22 shows a Tm value of 48°C. (c) The melting curve of the Sc-Rsm22 monomer shows a Tm value of 39°C. |
![[Figure 3]](jc5035fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
access
