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Figure 5
Example experiment for the methylation of mt-tRNAMet by monomeric Sc-Rsm22. In controls 1–3, either the enzyme Sc-Rsm22 was omitted (control 1), mt-tRNAMet was omitted (control 2) or the reaction mixture was treated with RNase (control 3). All three controls are missing at least one essential component for the reaction, preventing a specific methylation reaction. The measured dpm values for the different negative controls were in the range 47–145. The average dpm value for mt-tRNAMet methylation is 816.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 77| Part 6| June 2021| Pages 840-853
https://doi.org/10.1107/S2059798321004149
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