A simple vapor-diffusion method enables protein crystallization inside the HARE serial crystallography chip

Norton-Baker, B.; Mehrabi, P.; Boger, J.; Schönherr, R.; von Stetten, D.; Schikora, H.; Kwok, A.O.; Martin, R.W.; Miller, R.J.D.; Redecke, L.; Schulz, E.C.

doi:10.1107/S2059798321003855

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
Crystal centering using gentle centrifugation. The same features of a chip with lysozyme crystals are shown (a) before and (b) after centrifugation for 1 min. Hit maps for two identical chips prepared from the same stock solution of lysozyme:precipitant mixture (c) without centrifugation and (d) with centrifugation. Blue indicates a recorded diffraction pattern that was successfully indexed by CrystFEL.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 77| Part 6| June 2021| Pages 820-834

https://doi.org/10.1107/S2059798321003855

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