issue contents

Journal logoSTRUCTURAL
ISSN: 2059-7983

July 2021 issue

Highlighted illustration

Cover illustration: Crystal structure of Scone: pseudosymmetric folding of a symmetric designer protein [Mylemans et al. (2021), Acta Cryst. D77, 933–942]. Here, attempts were made to engineer a ninefold-symmetric β-propeller protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein.

topical reviews

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This article lists the basic crystallographic equations underlying molecular-replacement software. The prospects for future developments are considered, and a brief account of past progress is included.

research papers

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An intensity-based likelihood method is provided to estimate scattering from an anomalous substructure considering the effect of measurement errors in Bijvoet pairs and the correlations between these errors.

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The structure of PSK, a novel antimicrobial peptide identified in Chrysomya megacephala larvae, was determined by X-ray crystallography and small-angle X-ray scattering, which revealed a distinct fold compared with published homologous peptides.

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ParD2 forms an open oligomer in solution, the size of which is limited by the presence of an intrinsically disordered tail. In the absence of this tail, ParD2 forms a circular hexadecamer.

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The crystal structure of the ferredoxin reductase component of carbazole 1,9a-dioxygenase from Janthinobacterium sp. J3 was determined at 2.40 Å resolution.

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Using computational protein design, a symmetric protein was designed; however, its crystal structures revealed a different pseudosymmetric architecture. Furthermore, one variant was only able to crystallize upon the addition of a polyoxometalate which links individual proteins in the crystal packing.

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Coronaviruses encode a papain-like protease (PLP) which is responsible for antagonizing the innate immune response; here, the structure of the first known Alphacoronavirus PLP bound to ubiquitin, its preferred substrate, is reported.

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Based on the crystal structure of human fatty acid-binding protein FABP7 in complex with palmitic acid, the recognition of saturated fatty acids by FABP7 is discussed.

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An automated particle-picking method for cryo-EM and its evaluation using precision TEM simulation based on a multi-slice method are described.

addenda and errata

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