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![[Figure 1]](dw5221fig1mag.jpg)
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Figure 1
VcParE2 and VcParD2 are monodisperse, folded proteins. (a) CD spectra of 0.15 mg ml−1 VcParD2 (grey) and VcParE2-His (black) in 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM TCEP. (b) Analytical SEC elution profile of 0.15 mg ml−1 VcParE2 in 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM TCEP on a Superdex Increase 75 10/300 column. The elution volumes of the molecular-weight standards (chicken ovalbumin, 44 kDa; horse myoglobin, 17 kDa; vitamin B12, 1.35 kDa) are shown as diamonds together with a linear regression (black line). (c) Analytical SEC elution profile of 0.1 mg ml−1 VcParD2 and the VcParD2–VcParE2 complex in 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM TCEP on a Superdex Increase 200 10/300 column. The elution volumes of the molecular-weight standards (bovine γ-globulin, 158 kDa; chicken ovalbumin, 44 kDa; horse myoglobin, 17 kDa) are shown as diamonds together with a linear regression (black line). |
![[Figure 1]](dw5221fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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