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![[Figure 2]](dw5221fig2mag.jpg)
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Figure 2
VcParD2 is a specific DNA-binding protein. (a) EMSA experiment with VcParD2 titrated against a 151 bp DNA fragment upstream of the ATG start codon of ParD2. VcParD2 concentrations (in monomer equivalents) are given at the top in micromolar units. Binding is observed from 6 µM onwards and involves at least two discrete steps. (b) Equivalent EMSA using a 207 bp fragment derived from the operator sequence of the C. metallidurans psrQ2 gene. The VcParD2 concentrations used were identical to those in (a). No binding was observed for this fragment in the concentration range tested. |
![[Figure 2]](dw5221fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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