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Figure 7
SEC-MALS. (a) A typical SEC-MALS run (10 mg ml−1 VcParD2, 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM TCEP). The observed elution peak and plateau for molecular mass is representative of all conditions tested. (b) MALS-derived molecular weights as a function of concentration and experimental conditions. No clear dependence on concentration or ionic strength is observed. The molecular-mass values at pH 5.6 are nevertheless systematically higher than for the measurements at pH 8.0.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 77| Part 7| July 2021| Pages 904-920
https://doi.org/10.1107/S2059798321004873
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