Figure 9
Small-angle X-ray scattering. (a) Experimental SAXS data (black) overlaid with the theoretical scattering curve for a VcParD2 dodecamer averaged over the ten best-fitting conformations (red; χ2 = 2.11) and the curve calculated for the hexadecameric crystallographic assembly (cyan). (b) χ2 values for the best fits of octameric, decameric, dodecameric, tetradecameric and hexadecameric ensembles. A minimum is seen for a dodecameric ensemble. (c) Molecular model of the dodecameric ensemble that best represents the scattering data. The folded parts of VcParD2 dimers are alternately shown in blue and pink. The IDP tails are shown in grey. |