research papers
Janthinobacterium sp. J3
of the reductase component of carbazole 1,9a-dioxygenase fromaAgro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan, bAdvanced Analysis Center, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan, cDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan, dAgricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan, and eCollaborative Research Institute for Innovative Microbiology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
*Correspondence e-mail: anojiri@mail.ecc.u-tokyo.ac.jp
Carbazole 1,9a-dioxygenase (CARDO), which consists of an oxygenase component and the electron-transport components Janthinobacterium sp. J3 (CARDO-RJ3). Superimposition of the type I and type II structures revealed the absence of flavin adenine dinucleotide (FAD) in the type II structure along with significant conformational changes to the FAD-binding domain and the C-terminus, including movements to fill the space in which FAD had been located. Docking simulation of NADH into the FAD-bound form of CARDO-RJ3 suggested that shifts of the residues at the C-terminus caused the nicotinamide moiety to approach the N5 atom of FAD, which might facilitate between the redox centres. Differences in domain arrangement were found compared with RO reductases from the ferredoxin–NADP reductase family, suggesting that these differences correspond to differences in the structures of their redox partners and terminal oxygenase. The results of docking simulations with the redox partner class III CARDO-F from Pseudomonas resinovorans CA10 suggested that complex formation suitable for efficient is stabilized by electrostatic attraction and complementary shapes of the interacting regions.
(CARDO-F) and reductase (CARDO-R), is a Rieske nonheme iron oxygenase (RO). ROs are classified into five subclasses (IA, IB, IIA, IIB and III) based on their number of constituents and the nature of their redox centres. In this study, two types of (type I and type II) were resolved of the class III CARDO-R fromKeywords: Rieske nonheme iron oxygenase; NAD(P)H:ferredoxin oxidoreductase; ferredoxin; Janthinobacterium sp. J3; carbazole 1,9a-dioxygenase; CARDO; electron transfer.
1. Introduction
Rieske nonheme iron oxygenases (ROs) are the initial catalysts in the degradation pathways of numerous environmentally hazardous aromatic compounds and components of crude oil (Mason & Cammack, 1992; Nojiri & Omori, 2002; Nojiri, 2012). Few enzymes catalyse the introduction of O atoms into stable aromatic hydrocarbon substrates. ROs, one group of such enzymes, have been suggested to use a novel oxygen activation and addition mechanism. With very few exceptions, ROs catalyse the incorporation of both O atoms from molecular dioxygen onto tandemly linked C atoms of an aromatic ring, forming two hydroxyl groups in the cis configuration. ROs generally consist of two or three discrete components that form an electron-transfer chain from NAD(P)H via flavin and [2Fe–2S] redox centres to the site of dioxygen activation (Bugg & Ramaswamy, 2008). ROs have been classified into five groups, IA, IB, IIA, IIB and III, based on the number of constituents and the nature of their redox centres (Batie et al., 1991; Ferraro et al., 2005). Class I ROs consist of reductase and oxygenase components, with their reductase components containing both flavin [flavin mononucleotide (FMN) in class IA and flavin adenine dinucleotide (FAD) in class IB] and a chloroplast-type [2Fe–2S] cluster. Both class II and class III ROs contain a component in addition to reductase and oxygenase components. Class II is further divided into classes IIA and IIB, which use putidaredoxin-type and Rieske-type ferredoxins, respectively. Class II reductases contain FAD as the only cofactor, whereas class III reductases contain FAD and a chloroplast-type [2Fe–2S] cluster. Although there is variation in the redox-transfer machineries of both the reductase and the these components transfer electrons from NAD(P)H to oxygenase for dioxygen activation.
Carbazole 1,9a-dioxygenase (CARDO), which has been isolated from various carbazole-degrading bacteria, is an RO that catalyses the initial dioxygenation reaction in the carbazole-degradation pathway (Nojiri & Omori, 2007; Inoue et al., 2004, 2005; Vejarano et al., 2018, 2019). All reported CARDOs consist of three components: the terminal oxygenase CARDO-O, the CARDO-F and the reductase CARDO-R (Supplementary Fig. S1). CARDO-O is a homotrimeric enzyme that contains one Rieske-type [2Fe–2S] cluster and one active-site iron (Fe2+) in a single subunit. The electron-transport proteins of CARDO, which mediate electron transport from NAD(P)H to CARDO-O, comprise CARDO-F, which contains one Rieske-type [2Fe–2S] cluster, and CARDO-R, which contains one FAD and one plant-type [2Fe–2S] cluster. The CARDOs from Pseudomonas resinovorans CA10, Janthinobacterium sp. J3, Novosphingobium sp. KA1 and Nocardioides aromaticivorans IC177 are grouped into classes III, III, IIA and IIB, respectively (Sato et al., 1997; Inoue et al., 2004, 2006; Urata et al., 2006), indicating that their CARDOs include diverse types of electron-transfer components (CARDO-F and CARDO-R). Although the structures of several RO components are known (Ferraro et al., 2005; Senda et al., 2007; Lin et al., 2012), the precise nature of their electron-transfer mechanism remains unclear. Therefore, CARDOs provide an excellent model system for studying the structure–function relationships of RO components and the mechanism of electron transfer.
To date, crystal structures of the CARDO-F from P. resinovorans CA10 (CARDO-FCA10; Nam et al., 2005), CARDO-O from Janthinobacterium sp. J3 (CARDO-OJ3; Nojiri et al., 2005) and the electron-transfer complex between CARDO-OJ3 and CARDO-FCA10 (Ashikawa et al., 2005, 2006) have been identified. The structures of the class IIB CARDO-O and CARDO-F from N. aromaticivorans IC177 (CARDO-O177 and CARDO-FIC177) have also been determined (Inoue et al., 2009). However, no CARDO-R structures have yet been successfully determined. Considering that no class III RO reductase containing a plant-type [2Fe–2S] cluster and FAD has been structurally elucidated, structural and functional studies of a class III CARDO-R are needed to clarify the diversity of electron-transfer functions.
RO reductases belong to two distinct families: the ferredoxin–NADP reductase (FNR) family and the glutathione reductase (GR) family. The structures of phthalate dioxygenase reductase (PDO-R; class IA) from Pseudomonas cepacia PHK (Correll et al., 1992) and benzoate dioxygenase reductase (BZDO-R; class IB) from Acinetobacter baylyi ADP1 (Karlsson et al., 2002), both of which belong to the FNR family, have been determined. Both of these reductases are composed of a domain containing a plant-type [2Fe–2S] cluster, an FAD-binding domain and an NADH-binding domain. In addition, structures of the reductase components of biphenyl dioxygenase from Pseudomonas sp. KKS102 and of toluene dioxygenase from P. putida F1, which belong to the GR family, have been determined as examples of class IIB RO reductases (Senda et al., 2000; Lin et al., 2012). These reductases consist of three domains: an NADH-binding domain, an FAD-binding domain and a C-terminal domain corresponding to the interface domain in the GR family. In CARDO systems, the class III CARDO-RCA10 and CARDO-RJ3 belong to the FNR family, while the class IIB CARDO-RIC177 and the class IIA CARDO-R from Novosphingobium sp. KA1 (CARDO-RKA1) are classified into the GR family. Here, we report the of CARDO-RJ3, providing the first description of the of a class III RO reductase. This report makes the complete series of structures of all CARDO system components available for the first time. Using this newly determined structure, we investigated the binding of NADH to CARDO-RJ3 and the formation of the electron-transfer complex using docking simulations. Furthermore, we assessed the electron-transfer interaction between CARDO-R and its cognate CARDO-F using molecular-docking simulations. These results strengthen our understanding of how the interactions between discrete components can affect complex formation and in the RO system.
2. Materials and methods
2.1. Purification and crystallization
The Janthinobacterium sp. J3 (CARDO-RJ3) was expressed in Escherichia coli and purified as described previously (Ashikawa et al., 2007). The crystallization conditions used to obtain the two types of crystals have been described previously (Ashikawa et al., 2007). The selenomethionine (SeMet) substituent of CARDO-RJ3 was expressed in the methionine-auxotrophic E. coli strain B834 (DE3) (Novagen). E. coli strain B834 (DE3) transformed with pEJ3NAd was grown in medium containing SeMet (Doublié & Carter, 1992) and was purified and crystallized using the same methods as used for native CARDO-RJ3.
reductase component from2.2. Data collection
X-ray diffraction data were collected from the CARDO-RJ3 crystals at 100 K at a wavelength of 1.0 Å on beamline NW12A at the Photon Factory Advanced Ring, High Energy Accelerator Research Organization. Diffraction data were collected from the native crystals and processed with CrystalClear (Rigaku, Japan). The data-collection and processing statistics are given in Table 1.
‡R is defined as R = . §Rfree was calculated using 5% of the unique reflections. |
2.3. Single-wavelength and multi-wavelength anomalous diffraction (SAD and MAD) phasing
The structure of the type I CARDO-RJ3 crystal (Ashikawa et al., 2007) was determined by single-wavelength anomalous diffraction (SAD) and multi-wavelength anomalous diffraction (MAD) experiments using the of selenium in the SeMet-substituted crystal (peak 0.97945 Å) and of iron in the native crystal (peak 1.73974 Å, edge 1.73993 Å and remote 1.69243 Å), respectively (Table 1). The collected data were processed and scaled using HKL-2000 (Otwinowski & Minor, 1997). Both types of phase calculation were performed using SOLVE/RESOLVE (Terwilliger & Berendzen, 1999; Terwilliger, 2000) and SHARP/autoSHARP (Vonrhein et al., 2007). The positions of 18 strong selenium peaks (seven Se atoms per molecule) and three large iron peaks (two Fe atoms per molecule) were determined (figures of merit of 0.35 and 0.47, respectively). The initial phase obtained from the Fe-MAD experiment was not sufficient to construct the model structure, and the initial structure was determined by improving the phase using the Se-SAD data.
2.4. Structure refinement
Building of the initial model of the type I crystal from the electron-density map was carried out with QUANTA (Accelrys, San Diego, California, USA) and Coot (Emsley et al., 2010). was conducted using REFMAC5 in CCP4 (Winn et al., 2011) and CNS 1.1 (Brünger et al., 1998) through the gradual addition of water molecules. The type II structure was determined by with Phaser (McCoy et al., 2007) using the type I structure, and model building and were performed using the programs listed above. The stereochemistry of the model was analysed using PROCHECK (Laskowski et al., 1993), WHATCHECK (Hooft et al., 1996), RAMPAGE (Lovell et al., 2003) and MolProbity (Chen et al., 2010). The are listed in Table 1.
2.5. NAD(P)H docking simulation
CARDO-RJ3 was superimposed with pea FNR in complex with NADPH (PDB entry 1qfz; Deng et al., 1999) using Coot to build an initial model of NADH and NADPH binding. Some changes in the predicted positions of the NAD(P)H–CARDO-RJ3 complexes were performed considering protein–ligand interactions. After building the final models, the position and geometry of NAD(P)H in the CARDO-RJ3 structure were minimized using Phenix (Liebschner et al., 2019).
2.6. Docking simulations between CARDO-RJ3 and CARDO-FCA10
Docking simulation between CARDO-RJ3 and CARDO-FCA10 (PDB entry 1vck; Nam et al., 2005) was performed using the ClusPro web server (https://nrc.bu.edu/cluster; Comeau et al., 2004a,b). Rigid-body docking was performed with a scoring function based on shape complementarity, electrostatic potential and terms. Predictions for fitting the ligand protein (CARDO-FCA10) to the receptor protein (CARDO-RJ3) were then filtered using residues ligated to the [2Fe–2S] cluster of CARDO-FCA10 and a 6 Å distance cutoff, and were clustered (9 Å clustering radius) and ranked using the automated ClusPro web server. The ligand protein with the greatest number of neighbours was the cluster centre, and this number was minimized using the CHARMM algorithm in the presence of the receptor protein.
3. Results and discussion
3.1. Quality of structures
In a previous study, we obtained two types of CARDO-RJ3 crystals (type I and type II; Ashikawa et al., 2007). for the two crystal structures of CARDO-RJ3 are summarized in Table 1. The type I crystals were resolved using SAD (Se atoms) and MAD (Fe atoms) experiments. The type I structure was refined at 2.6 Å resolution with a crystallographic R factor of 0.249 (Rfree = 0.292; Fig. 1a). The type I structure contained three CARDO-RJ3 molecules per (Supplementary Fig. S2a); one residue (Leu289 in chain C) fell into the outlier region of the Ramachandran plot created using RAMPAGE (Lovell et al., 2003). Superposition of the three molecules yielded a root-mean-square deviation (r.m.s.d.) value of 0.79 Å for 313 Cα atoms. The type II was determined by the molecular-replacement method using the type I structure and was refined at 2.4 Å resolution with a crystallographic R factor of 0.227 (Rfree = 0.281; Fig. 1b). The contained three CARDO-RJ3 polypeptide chains, as in the type I structure. One residue, Leu289 in chain B, fell outside the allowed regions of the Ramachandran plot (Lovell et al., 2003). Superposition of the three molecules yielded an r.m.s.d. value of 0.88 Å for 301 Cα atoms. The three molecules in the of each structure were connected by a noncrystallographic threefold axis, although CARDO-RJ3 was a monomer in solution. In both structures, electron density for one Ni2+ ion, which was deduced by analyses using the anomalous Fe-MAD map and CheckMyMetal (Zheng et al., 2014), was observed on this axis and coordinated by two histidine residues of the N-terminal His tag from each of the three molecules (Supplementary Fig. S2b). Some disordered regions were present in all molecules in both structures. The type I structure contained the [2Fe-2S] cluster and FAD, while the type II structure lacked FAD (the apo form), which is essential for the physiological function of CARDO-RJ3. In addition, in both structures we observed chloride ions and iodine ions, which were contained in the crystallization buffer and possess larger electron densities compared with water molecules (Supplementary Fig. S2c). Both structures did not contain NAD(P)H.
3.2. Overall structure of CARDO-RJ3
CARDO-RJ3 is a 329-residue monomeric enzyme that belongs to the FNR superfamily. It consists of three domains: an N-terminal (Fd) domain (residues 1–99), an FAD-binding domain (residues 100–196) and an NADH-binding domain (residues 197–329) (red, yellow and blue regions, respectively, in Fig. 2). The N-terminal Fd domain contains the plant-type [2Fe–2S] cluster. The FAD-binding domain is in a central position in the molecule and interacts with the Fd domain on one side and the NADH-binding domain on the other side; the Fd and NADH-binding domains have little direct interaction.
On superimposing the type I and type II structures, conformational differences were observed in several regions of the FAD-binding domain (Fig. 3). Residues 165–183 in the apo type II structure lacking FAD shifted greatly towards the FAD-binding site to fill space, and the α-helix of residues 175–180 was not formed. Two loops (residues 114–116 and 139–148) moved to fit into the shifted region at residues 165–183. Amino-acid residues in these regions formed hydrogen bonds to FAD, which may be important for accurate binding of FAD. Interestingly, a significant difference was found in the locations of three C-terminal amino-acid residues (Ala327, Phe328 and Phe329) between the two structures (Fig. 3). In particular, movement of the side chain of Phe328 was required to make space for the isoalloxazine ring of FAD, suggesting high flexibility in this region. For further structural comparison we used the type I structure of CARDO-RJ3, as the FAD-bound form exhibits activity as an electron-carrier protein.
3.3. (Fd) domain
In CARDO-RJ3, the Fd domain consists of an acutely twisted β-sheet and three short α-helices. The overall folding of the Fd domain showed high similarity to plant-type ferredoxins (Fukuyama, 2004). The Fd domain contains a [2Fe–2S] cluster coordinated by four cysteine residues in the Cys-X4-Cys-X2-Cys-Xn-Cys motif (Rypniewski et al., 1991). In CARDO-RJ3, Cys35, Cys40, Cys43 and Cys76 coordinate the Fe atoms of the [2Fe–2S] cluster, while the main-chain N atoms of residues 34, 36 and 38–41 form hydrogen bonds to the S atoms in the cluster (Figs. 4a and 4b). In addition, the cluster is surrounded by the side chains of Tyr33 and Leu74, which create a hydrophobic environment (Fig. 4a).
In the CARDO-RJ3 structure, the position of the main-chain O atom of Cys35 (Cys35 O) was closest to the methyl group at the C8 position (C8M) of FAD (average distance of 3.3 Å), suggesting that this orientation would be preferable for between the two redox centres. The assumption that these two atoms are likely to take part in the electron-transfer reaction is supported by the results obtained from calculations using HARLEM, a program for predicting electron-transfer pathways (Kurnikov, 2003). However, we must consider the possibility that this orientation may be altered by the binding of NAD(P)H.
On the other hand, comparison of the structural configuration of Cys40 O in CARDO-RJ3 with those of other plant-type ferredoxins indicated that the orientation of Cys40 O is most similar to that associated with the [2Fe–2S] cluster in the one-electron-reduced state, as observed in putidaredoxin (Sevrioukova, 2005) and T4moF (Acheson et al., 2015) (Supplementary Fig. S3). It is possible that the cluster may be reduced by from synchrotron radiation because Cys40 O faces `out' even though the crystals of CARDO-RJ3 were prepared under aerobic conditions.
3.4. FAD-binding domain
The FAD-binding domain of CARDO-RJ3 is similar to those of BZDO-R (Karlsson et al., 2002) and T4moF (Acheson et al., 2015), which are smaller than the corresponding domains in other members of the FNR-like superfamily (Bruns & Karplus, 1995). The domain is mainly made up of a six-stranded β-sheet and an α-helix, which form a cleft into which the isoalloxazine and ribityl moieties of FAD can bind (Figs. 1a and 2).
The relationship between FAD and the [2Fe–2S] cluster in the Fd domain, including important coordinated residues around the redox centre, is shown in Fig. 4(b). Residues in the FAD-binding domain (Ser151, Tyr165, Lys167 and Ser217) form hydrogen bonds to the FAD isoalloxazine ring (Fig. 4c). Phe135 in the FAD-binding domain and Phe328 in the NADH-binding domain exhibit π-stacking interactions, and hydrophobic interactions with Ala149 stabilize the FAD isoalloxazine ring, sandwiching it from the top and bottom (Figs. 4b and 4c). Therefore, the average B factors of the FAD isoalloxazine ring (53 Å2) were lower than those of the rest of the FAD molecule (70 Å2). The C-terminus of the NADH-binding domain is involved in FAD binding in most FNR-like proteins. Phe328 is homologous to Phe325 in T4moF, Phe335 in BZDO-R, Phe225 in PDO-R and Tyr314 in maize leaf FNR–Fd (Fig. 2), all of which exhibit a π-stacking interaction with an aromatic residue on the isoalloxazine ring of flavin (Figs. 4b and 4c).
Two important interactions occur between the protein and the adenine moiety of FAD in the FAD-binding region: a cation–π interaction (Arg148 of the FAD-binding domain) and a π-stacking interaction (Trp56 of the Fd domain), as observed in T4moF (Fig. 4b). This contribution of the Fd domain is unique compared with observations on other FNRs [maize leaf FNR–Fd (PDB entry 1gaq), Anabaena FNR–Fd (PDB entry 1ewy) and BZDO-R (PDB entry 1krh)], where the adenine moiety-stabilizing interactions are supported either by the FAD-binding domain alone or by cooperation between the FAD- and NADH-binding domains (Kurisu et al., 2001; Morales et al., 2000; Karlsson et al., 2002). Although the CARDO-RJ3 and BZDO-R components are both involved in RO, a notable difference exists in the role of the Fd domains in FAD binding, which might be caused by their distinctive electron-transfer partners: the Fd component for CARDO-RJ3 and the oxygenase component for BZDO-R. However, the average B factors for the adenine moiety of FAD (81 Å2) were higher than those for the rest of FAD (60 Å2), suggesting that the adenine moiety was not tightly bound.
3.5. NADH-binding domain
The NADH-binding domain consists of a five-stranded β-sheet surrounded by five α-helices, which is typical of the FNR-like superfamily (Ingelman et al., 1997). In CARDO-RJ3, π-stacking occurs between the side chain of the penultimate Phe328, in the NADH-binding domain and the isoalloxazine ring of FAD, while the C-terminal tyrosine residues are involved in this interaction in photosynthetic FNRs such as maize leaf FNR–Fd (Figs. 2, 4b and 4c; Karplus et al., 1991; Serre et al., 1996). In addition, as shown in Fig. 4(c), Ser217 in the NADH-binding domain forms a hydrogen bond to the O4 atom of the isoalloxazine ring on the same side as in PDO-R (Correll et al., 1992).
Even though extensive attempts were made to determine the structure of CARDO-RJ3 bound to NADH or NADPH, co-crystallization of CARDO-RJ3 with either NADH or NADPH failed to produce crystals, and NAD(P)H soaking caused the rapid dissolution of crystals. These results appear to be reasonable, as other researchers have reported that the binding of NAD(P)H promoted conformational rearrangement in this family of reductases (Correll et al., 1993). In fact, the CARDO-RJ3 structure suggests that the nicotinamide moiety of NAD(P)H must be replaced with Phe328 from the NADH-binding domain, which interacts with the isoalloxazine ring, for the hydride-transfer reaction to occur (Fig. 4b).
In previous studies, both NADH and NADPH were effective electron donors for CARDO-RCA10 (Nam et al., 2002). To obtain essential structural insights into the binding of CARDO-RJ3 by NAD(P)H, pea FNR bound to NADPH (PDB entry 1qfz) was aligned with CARDO-RJ3 as a template, and NAD(P)H was then incorporated into the modelled CARDO-RJ3 structure. Additional adjustment of the position of NAD(P)H in CARDO-RJ3 was carried out considering the interactions around various residues using Coot (Emsley et al., 2010). After building the final models, energy minimization was performed using Phenix (Liebschner et al., 2019; Fig. 5).
The docking model with NADH revealed that the adenine moiety fits into the cleft between Phe279 and Pro304, while Arg241 coordinates to the pyrophosphate moiety. In addition, Thr266 is likely to form hydrogen bonds to the O2B and O3B atoms of the ribose moiety of NADH. The phenyl ring of Phe328 shifts greatly towards the Fd domain (about 3–5 Å) so that the C4N atom of the nicotinamide moiety is located near the FAD N5 atom to promote the transfer of hydride to FAD. The hydride-transfer reaction is also facilitated by polarization of FAD N1, which is provided by a hydrogen-bonding network involving Ser151, Ser217, Lys167 and the FAD O4 and O2 atoms (Fig. 4c). Notably, docking of NADH causes a large shift in the positions of the C-terminal residues around Phe328 (Asp326, Ala327 and Phe329), which generates a new hydrogen-bonding interaction with Glu34 in the Fd domain (Fig. 5). Incidentally, the NADH-binding domain moves slightly towards the Fd domain. These conformational changes might facilitate between the redox centres, FAD and the [2Fe–2S] cluster. In addition, the FAD- and NADH-binding domains appear to move slightly apart in the model with NADH, which has previously been reported in other FNR-type proteins, for example PDO-R (Correll et al., 1992).
The NADPH-bound model showed similar conformational changes to the NADH-bound model (data not shown). Arg241 forms hydrogen bonds not only to the pyrophosphate moiety, but also to the phosphate moiety, which contributes to a greatly increased stability of NADPH binding. This result suggests that both NADH and NADPH act as electron donors for CARDO-RJ3.
3.6. Comparison with other reductases
The structure of CARDO-RJ3 is the first reported structure of a class III reductase, which can be compared with those of PDO-R (class IA; Correll et al., 1992) and BZDO-R (class IB; Karlsson et al., 2002) among the ROs. Through sequence comparison, CARDO-RJ3, BZDO-R and PDO-R were found to share the same three domains, the Fd, FAD-binding and NAD-binding domains, but these three domains are ordered differently in the proteins (Fig. 2). The FAD-binding domain is followed by the NADH-binding domain in the amino-acid sequence, but the Fd domain is connected to the C-terminus of the NADH-binding domain in PDO-R and to the N-terminus of the FAD-binding domain in CARDO-RJ3 and BZDO-R. The structures of PDO-R and CARDO-RJ3/BZDO-R demonstrate that these three domains are positioned similarly despite their different locations in the sequence. On the other hand, on comparing the sequence and structure of T4moF (Sevrioukova, 2005; Acheson et al., 2015), the order and structural positions of the domains are the same in T4moF, CARDO-RJ3 and BZDO-R.
A detailed comparison of the structures of CARDO-RJ3 and the BZDO-R (PDB entry 1krh), PDO-R (PDB entry 2pia) and T4moF (PDB entry 4wqm) was carried out. Superposition of the Fd domains revealed strong agreement, particularly between CARDO-RJ3 and T4moF, which aligned with an r.m.s.d. of 1.37 Å2 (on 97 Cα atoms). Likewise, comparison of the FNR-like domains (including both the FAD- and NADH-binding domains) among the four proteins showed r.m.s.d. values of 1.67–2.48 Å2. These comparisons indicate that there were minor structural differences among the individual domains.
Alignment of the FNR-like domains (the right parts of the molecules shown in Fig. 6) of CARDO-RJ3 with BZDO-R (Fig. 6a), PDO-R (Fig. 6b) and T4moF (Fig. 6c) suggested an interesting difference in the relative positions of the Fd domains in their overall structures. The relative positioning of the Fd domains in relation to the other two domains (FNR-like domains) differed significantly, except between CARDO-RJ3 and T4moF, despite the [2Fe–2S] clusters being located in similar positions. The positions of the [2Fe–2S] clusters in the Fd domains were likely to be constrained by the requirement for efficient between the flavin cofactor and the [2Fe–2S] cluster. The distance between Cys35 O, the cysteine residue in the plant-type [2Fe–2S] motif, and the C8M atom of the FAD domain (suggested to be the preferred contact site for electron transfer) in CARDO-RJ3 was 3.3 Å (Fig. 4b), which is similar to that in T4moF (3.7 Å). On the other hand, the O atoms of the cysteine residues were located within distances of 5.4 and 4.7 Å in PDO-R and BZDO-R, respectively. Investigation of the crystal-packing patterns of the four protein structures suggested that crystal packing did not affect the positions of the Fd domains (data not shown). In PDO-R and BZDO-R, the positions of the Fd domains were shifted to make space for the possible binding of their redox-partner proteins, i.e. the oxygenase components. In PDO-R, this space was a flat surface containing the Fd domain and the NADH-binding domain, while in BZDO-R the position of the Fd domain left space on the other side near the FAD-binding domain (Karlsson et al., 2002; Fig. 7). Such differences in the space available for binding may be due to the differing configurations of the oxygenases, which are a homotrimer (α3) and a heterotrimer (α3β3) for the PDO and BZDO systems, respectively. On the other hand, CARDO-RJ3 and T4moF have similar clefts interposed by Fd and NADH-binding domains, which may fit the arrowhead shape of the relatively small protein (Fig. 7). These differences are likely to correspond to complementary differences in the structures of their respective redox partners: and terminal oxygenase.
3.7. Modelled complex of CARDO-RJ3 and CARDO-FCA10 in the class III CARDO system
We assessed the binding position of the electron-transfer partner CARDO-FCA10 on the structure obtained for CARDO-RJ3 using the protein–protein docking simulation software ClusPro (Comeau et al., 2004a,b). Docking simulations provided several plausible structures for the CARDO-R–CARDO-F complex among a large number of solutions. One of these plausible structures is shown in Fig. 8(a). CARDO-FCA10 was bound to the cleft between the Fd and NADH-binding domains of CARDO-RJ3, resulting in remarkably good steric and electrostatic matching, as described below. Notably, the docked structure placed the [2Fe–2S] clusters of CARDO-FCA10 and CARDO-RJ3 ∼11.0 Å from each other, which is within the predicted suitable distance range for biological (Page et al., 1999). This finding suggests that between these redox partners is possible under the binding conditions shown in Fig. 8(a). As shown in Figs. 8(b) and 8(c), the minimum-energy solution showed some interesting features. Several electrostatic and hydrophobic interactions were observed in the proposed complex. An examination of the electrostatic surface potentials of CARDO-RJ3 and CARDO-FCA10 revealed that Coulomb attraction between the two proteins is likely to stabilize the complex. The interface between the two proteins is established in a region that is positively charged on the CARDO-RJ3 side (Lys44, Arg65, Lys69 and Arg72 in the Fd domain) and negatively charged on the CARDO-FCA10 side (Asp59, Asp61 and Glu64) (Figs. 8b and 8c). Meanwhile, on the side opposite the static interaction described above, hydrophobic interactions were observed between CARDO-RJ3 (Val310, Leu314 and Phe325 in the NADH-binding domain) and CARDO-FCA10 (Ile50 and Phe67) (Fig. 8c). Small electron-carrier proteins, such as CARDO-FCA10, participate in electron shuttling between the donor and acceptor sites through diffusive encounters and the formation of transient protein complexes (Crowley & Ubbink, 2003; Crowley & Carrondo, 2004). Without appropriate docking to bring the redox centres closer and the formation of a long-range electron-transfer route, it has been suggested that diffusional encounters between the two electron-transfer partners would dramatically slow complex formation and (Page et al., 1999). In the CARDO-RJ3–CARDO-FCA10 complex, sufficient electrostatic and structural matching between the two partners (Fig. 8) would allow efficient As noted above, between FAD and the [2Fe–2S] cluster may proceed via FAD C8M and Cys35 O, as predicted by HARLEM (Kurnikov, 2003; Fig. 4b). In the predicted complex, subsequent from the [2Fe–2S] cluster of CARDO-RJ3 to the Rieske-type [2Fe–2S] cluster in CARDO-FCA10 was predicted to occur via Cys40 O and His68 N, coordinating the ligands for each cluster (Fig. 8c). The stable complex formation with sufficient electrostatic and structural matching described above ensures the presumed electron-transfer pathway, which can be assumed to lead to effective electron transfer.
4. Conclusions
This study of CARDO-RJ3 provides the first reported structure of a reductase from the class III RO family. The structure obtained here reveals differences in domain arrangement among reductases with different redox partners in their enzyme complexes within the RO family. In addition, based on the results of docking simulations with the redox partner, sufficient electrostatic attraction and shape matching of the interacting regions in the complex could enable efficient in the class III RO system.
On the other hand, despite low sequence identity and various permutations of the Fd and FNR-like domains, a high degree of structural homology is observed within the iron–sulfur flavoprotein family to which RO-system reductases belong (Karlsson et al., 2002). are generally considered to have optimized interactions with their cognate protein partners resulting from specific electrostatic and steric interactions among the residues within each electron-transport surface. The present study provides a new example of how interactions between proteins can promote complex formation and electron transfer.
Supporting information
Supplementary Figures. DOI: https://doi.org/10.1107/S2059798321005040/ji5017sup1.pdf
Footnotes
‡Present address: Education and Research Support Section, Technology Management Division, Administration and Technology Management Center for Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
§Current position: Professor Emeritus, The University of Tokyo.
Acknowledgements
The authors thank Professor Atsuko Yamashita of Okayama University for helpful advice. The data-collection procedure was approved by the Photon Factory Advisory Committee and the High Energy Accelerator Research Organization (KEK; proposal Nos. 2005G060, 2006G171, 2015G625 and 2019G102), as well as the Japan Synchrotron Radiation Research Institute (JASRI; proposal Nos. 2005A0671 and 2005B0985).
Funding information
Part of this work was supported by a Grant-in-Aid for Scientific Research (17380052 and 20248010 to HN) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by the Institute for Bioinformatics Research Development, Japan Science Technology Agency (BIRD-JST).
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