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![[Figure 4]](qg5001fig4mag.jpg)
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Figure 4
CCD2 offers multiple choices for primer generation. (a) CCD2 is (optionally) integrated with the pETNKI LIC series of vectors, which offer great versatility, since the same construct fits multiple vectors. (b) CCD2 can design primers for conventional restriction cloning. (c) CCD2 allows the choice of any custom overhang for primers. (d) CCD2 automatically generates primers based on user-chosen boundaries on the protein sequence, melting temperature and primer overhangs. Shown here are the primers for start positions 1 and 48 and stop positions 48 and 58 (from Fig. 3 ![[link]](../../../../../../logos/arrows/d_arr.gif) ), with a Tm of 65°C and overhangs for pETNKI LIC 1.1. The overhang portion of the primer is shown in lower case and the annealing portion is shown in upper case. The primers are named prefix_Fw/Rv_position, where the prefix is chosen by the user (for example RBX5), Fw stands for forward, Rv stands for reverse and `position' is the chosen start/stop position. Primers can be copied and pasted into a spreadsheet or saved in comma-separated value (csv) format. The DNA sequences of the constructs resulting from all possible combinations of primers can also be saved in csv format. |
![[Figure 4]](qg5001fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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