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Figure 5
Primer generation and protein-construct analysis. (a) The possible constructs resulting from the primers chosen in Fig. 3[link] and displayed in Fig. 4[link](e) are shown here, together with their predicted molecular wight (MW), isoelectric point (pI) and extinction coefficient ɛ at 280 nm. (b) For pETNKI vectors (for example pETNKI 1.1), the tagged and protease-cleaved constructs are also shown, together with their predicted physical properties.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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