Figure 5
Primer generation and protein-construct analysis. (a) The possible constructs resulting from the primers chosen in Fig. 3 and displayed in Fig. 4(e) are shown here, together with their predicted molecular wight (MW), isoelectric point (pI) and extinction coefficient ɛ at 280 nm. (b) For pETNKI vectors (for example pETNKI 1.1), the tagged and protease-cleaved constructs are also shown, together with their predicted physical properties. |