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Figure 10
Fluorescence polarization assay of DNA binding by STF-HD proteins. Fluorescent polarization is represented by millipolarization units (mP). The Kd values for each of the STF-HD proteins were calculated by nonlinear regression and standard deviations from triplicate values. The F142Y/Y143N mutant had a higher Kd value, indicating weaker binding to the DNA probe.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 77| Part 8| August 2021| Pages 1050-1063
https://doi.org/10.1107/S205979832100632X
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