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Figure 1
Single-particle analysis of BfrB. (a) A micrograph of a highly concentrated (80 mg ml−1) BfrB sample in vitreous ice collected on a Falcon 3 at 200 kV. (b) 2D class averages; the size of the shown box is 150 Å. (c) 3D reconstruction from 163 568 particles at 2.12 Å resolution collected on a K3 at 300 kV. (d) Gold-standard Fourier shell correlation (FSC) before (red line) and after (orange line) masking and the phase-randomized FSC (black line). |
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