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Figure 1
TrEM of actomyosin dissociation. (a) Schematic of the TED with different delay times indicated. (b) Microscopic image of the microfluidic gas dynamic virtual nozzle with mixing element used in this work. (c) Oscilloscope recording for a grid prepared with a stop of 600 ms between spray application and vitrification. The measured voltage corresponds to the vertical position of the grid. (d) Raw cryo-EM images of the actomyosin complex mixed with ATP and vitrified after 7, 13, 340 and 640 ms. The scale bar corresponds to 20 nm. (e) Consensus reconstructions of the actomyosin complex 7, 13, 340 and 640 ms after on-grid mixing with ATP, all shown at a 3σ threshold.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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