Figure 2
TrEM data processing. Reconstructions of F-actin (a) and the actomyosin complex (b) from the combined data. The central three actin subunits are shown in green and myosin S1 is in yellow. (c) Relative particle numbers of the actomyosin complex traced back to individual time points. Shown is the percentage of actomyosin particles relative to the total number of myosin-binding sites (the number of actin subunits). For each time point, the mean and standard deviation are shown as black lines and per-micrograph particle numbers are shown as grey points. |