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![[Figure 6]](qt5002fig6mag.jpg)
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Figure 6
Binding-affinity estimation between PknG and AX2017. (a) Fluorescence-based melting curves of PknG mixed with different concentrations of AX2017. At 150 µM, the rightmost yellow curve was observed. At 0.068 µM, the leftmost blue curve was observed. (b) Binding-affinity (Kd) estimation at 44°C, based on the coupling between the unfolding and binding equilibriums. (c) Normalized signal versus time. The `cold' (−1 to 0 s) and `hot' (0 to 2 s) regions are depicted by light blue and pink rectangles, respectively. (d) Fitting of the Fnorm versus ligand concentration curve. The experimental data points are represented by blue dots and the fitting by an orange line. |
![[Figure 6]](qt5002fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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