Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR

Luchinat, E.; Barbieri, L.; Cremonini, M.; Pennestri, M.; Nocentini, A.; Supuran, C.T.; Banci, L.

doi:10.1107/S2059798321009037

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

IUCr IT WDC

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Figure 2
Overlay of ¹H–¹⁵N NMR spectra of (a) pure [¹⁵N]-labeled CA II and (b) cells expressing [¹⁵N]-His-labeled CA II either in the absence of ligands (black) or bound to AAZ (red), MZA (green), SLC (blue) and ETZ (magenta). The signals of the free protein are labeled with the corresponding residue number and atom type (Shimahara et al., 2007