Figure 3
Closed-tube in-cell 1H–15N NMR spectra of cells treated with (a) 50 µM AAZ + 100 µM MZA, (b) 50 µM MZA + 100 µM SLC and (c) 50 µM AAZ + 100 µM SLC. Signals arising from CA II-bound AAZ, MZA and SLC are indicated with blue, black and red arrows, respectively. Only the least overlapped signals (marked with asterisks) were integrated for nonlinear fitting (see Supplementary Fig. S3). |