view article

Figure 4
Bioreactor in-cell NMR of cells treated with constant 10 µM MZA and increasing amounts of SLC (bioreactor run 1 in Table 1[link]) and subsequent data analysis. (ac) Representative 1H–15N NMR spectra at different time points and concentrations of SLC. Signals arising from SLC are marked with red arrows. (d) NMR spectra of the pure components, i.e. CA II–MZA (black) and CA II–SLC (red), reconstructed by MCR-ALS. (e) Concentration profiles of CA II–MZA (black squares) and CA II–SLC (red circles) obtained by MCR-ALS. (f) Bound fractions obtained from the plateau values after each step of the run plotted as a function of SLC concentration. Binding curves from nonlinear fitting are shown as dashed lines.

Journal logoSTRUCTURAL
ISSN: 2059-7983
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds